Investigation of nuclear DNA content and cell cycle phases in rat liver cells under chlorpromazine administration
نویسندگان
چکیده
Hepatotoxicity of antipsychotic drugs remains an urgent problem modern medicine. Therefore, the purpose study was to investigate nuclear DNA content and cell cycle phases rat liver cells under Сhlorpromazine administration at doses ranging from 3.5 mg/kg 28 for 30 60 days. The conducted on sexually mature female rats. Chlorpromazine administered once daily days mg/kg, 7 14 21 mg/kg. in nuclei determined by flow cytometry. Cytological analysis performed using FloMax software (Partec, Germany), where percentage G0G1 interval cycle, S phase, G2M interval, apoptosis index – SUB-G0G1 area histograms were determined. Statistical processing results Mann-Whitney U test. showed that has a dose-dependent hepatotoxic effect: with increase dose this drug rats fragmented tissue significantly increased, which is sign hepatocyte death apoptosis. It found did not apoptosis, while highest hepatotoxicity, increasing level 1.9 2.1 (p˂0.05) times, respectively. effect enhanced use days, manifested significant fragmentation, which, our opinion, should be taken into account when conducting long-term therapy patients.
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اثرات گاز کلر بر سیکل سلولی و محتوی DNA گلبول‌های سفید موش سوری The Effects of Chlorine on Cell Cycle and DNA Content of WBC in Mice
Background & Aim: Chlorine has been known as a mucus membranes and respiratory tract irritant. This gas can increase free radicals which cause cell damage. The aim of the present study was to measure DNA content and cell cycle in white blood cells after chronic chlorine poisoning. Materials & Methods: A clinical experimental study was carried out on 80 male mice(40 mice as sample and 40 as ...
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In individual altered hepatic foci (AHF), aneuploidy occurs before malignant changes can be diagnosed histologically (O. Sudilovsky and T. K. Hei. Fed. Proc., 42: 2225, 1983). In the current experiments SpragueDawley rats of both sexes were given i.p. injections of diethylnitrosamine (50 mg/kg body weight) 18 h after partial hepatectomy and were given a choline-sufficient diet (CS) for 1 wk. Fo...
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In individual altered hepatic foci (AHF), aneuploidy occurs before malignant changes can be diagnosed histologically (O. Sudilovsky and T. K. Hei. Fed. Proc., 42:2225, 1983). In the current experiments Sprague-Dawley rats of both sexes were given i.p. injections of diethylnitrosamine (50 mg/kg body weight) 18 h after partial hepatectomy and were given a choline-sufficient diet (CS) for 1 wk. Fo...
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ژورنال
عنوان ژورنال: Reports of Morphology
سال: 2023
ISSN: ['1818-1295', '2616-6194']
DOI: https://doi.org/10.31393/morphology-journal-2023-29(3)-04